Characterization of the carcinoma-associated Tk antigen in helminth parasites

Luis Ubillos; Andrea Medeiros; Martín Cancela; Cecilia Casaravilla; Jenny Saldaña; Laura Domínguez; Carlos Carmona; Jacques Le Pendu; Eduardo Osinaga

doi:10.1016/j.exppara.2006.12.009

Characterization of the carcinoma-associated Tk antigen in helminth parasites

Luis Ubillos, Andrea Medeiros, Martín Cancela, Cecilia Casaravilla, Jenny Saldaña, Laura Domínguez, Carlos Carmona, Jacques Le Pendu, Eduardo Osinaga

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Expression of Tk antigen, a truncated carbohydrate antigen, was examined in helmith parasites. Using the monoclonal antibody LM389, this antigen was detected in extracts from Taenia hydatigena, Mesocestoides vogae (syn corti), and Taenia crassiceps. No reactivity was observed in Thysanosoma spp., Dipylidium caninum, Fasciola hepatica, and Nyppostrongylus brasiliensis. On the basis of their electrophoretic mobility, different patterns of Tk-bearing glycoproteins were observed among T. hydatigena, M. corti and T. crassiceps by immunoblotting, with certain components resolved as broad bands typical of mucin-like glycoproteins. Most Tk-reactive material remained in the 0.6 N perchloric acid-soluble fraction, confirming that Tk epitopes are carried by mucin-type glycoproteins. Immunohistochemical analysis revealed that in T. hydatigena, Tk antigen is mainly expressed in the tegument, whereas in M. corti the reactivity was principally observed in the subtegumental parenchyma. The presence of a novel tumor-associated carbohydrate antigen in invertebrates, contributes to strengthen the notion that truncated mucin-type O-glycosylation is a normal phenomenon in parasitic worms and may help identify new biological characteristics of helminth parasites.

Original language	English
Pages (from-to)	129-136
Number of pages	8
Journal	Experimental Parasitology
Volume	116
Issue number	2
DOIs	https://doi.org/10.1016/j.exppara.2006.12.009
State	Published - Jun 2007
Externally published	Yes

Keywords

0.5% gelatin, 0.1% Tween 20 in phosphate buffered saline (PBS/TG)
Bovine serum albumin (BSA)
Carbohydrate cryptic antigen
Carbohydrate cryptic antigens
Deoxycholic acid (DOC)
Ethylenediaminetetracetic acid (EDTA)
Helminth parasites
Monoclonal antibody (MAb)
Mucin-type tumor markers
N-acetylgalactosamine (GalNAc)
O-glycosylation
Perchloric acid (PCA)
Phenylmethanesulfonyl fluoride (PMSF)
Phosphate buffered saline (PBS) (composition: 137 mM NaCl, 3 mM KCl, and 15 mM Na/K-phosphate buffer, pH 7.4)
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
Tk antigen
Tris-buffered saline (TBS) (composition: 150 mM NaCl, 50 mM Tris, pH 7.5)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.exppara.2006.12.009

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title = "Characterization of the carcinoma-associated Tk antigen in helminth parasites",

abstract = "Expression of Tk antigen, a truncated carbohydrate antigen, was examined in helmith parasites. Using the monoclonal antibody LM389, this antigen was detected in extracts from Taenia hydatigena, Mesocestoides vogae (syn corti), and Taenia crassiceps. No reactivity was observed in Thysanosoma spp., Dipylidium caninum, Fasciola hepatica, and Nyppostrongylus brasiliensis. On the basis of their electrophoretic mobility, different patterns of Tk-bearing glycoproteins were observed among T. hydatigena, M. corti and T. crassiceps by immunoblotting, with certain components resolved as broad bands typical of mucin-like glycoproteins. Most Tk-reactive material remained in the 0.6 N perchloric acid-soluble fraction, confirming that Tk epitopes are carried by mucin-type glycoproteins. Immunohistochemical analysis revealed that in T. hydatigena, Tk antigen is mainly expressed in the tegument, whereas in M. corti the reactivity was principally observed in the subtegumental parenchyma. The presence of a novel tumor-associated carbohydrate antigen in invertebrates, contributes to strengthen the notion that truncated mucin-type O-glycosylation is a normal phenomenon in parasitic worms and may help identify new biological characteristics of helminth parasites.",

keywords = "0.5\% gelatin, 0.1\% Tween 20 in phosphate buffered saline (PBS/TG), Bovine serum albumin (BSA), Carbohydrate cryptic antigen, Carbohydrate cryptic antigens, Deoxycholic acid (DOC), Ethylenediaminetetracetic acid (EDTA), Helminth parasites, Monoclonal antibody (MAb), Mucin-type tumor markers, N-acetylgalactosamine (GalNAc), O-glycosylation, Perchloric acid (PCA), Phenylmethanesulfonyl fluoride (PMSF), Phosphate buffered saline (PBS) (composition: 137 mM NaCl, 3 mM KCl, and 15 mM Na/K-phosphate buffer, pH 7.4), Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Tk antigen, Tris-buffered saline (TBS) (composition: 150 mM NaCl, 50 mM Tris, pH 7.5)",

author = "Luis Ubillos and Andrea Medeiros and Mart{\'i}n Cancela and Cecilia Casaravilla and Jenny Salda{\~n}a and Laura Dom{\'i}nguez and Carlos Carmona and \{Le Pendu\}, Jacques and Eduardo Osinaga",

year = "2007",

month = jun,

doi = "10.1016/j.exppara.2006.12.009",

language = "Ingl{\'e}s",

volume = "116",

pages = "129--136",

journal = "Experimental Parasitology",

issn = "0014-4894",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Characterization of the carcinoma-associated Tk antigen in helminth parasites

AU - Ubillos, Luis

AU - Medeiros, Andrea

AU - Cancela, Martín

AU - Casaravilla, Cecilia

AU - Saldaña, Jenny

AU - Domínguez, Laura

AU - Carmona, Carlos

AU - Le Pendu, Jacques

AU - Osinaga, Eduardo

PY - 2007/6

Y1 - 2007/6

N2 - Expression of Tk antigen, a truncated carbohydrate antigen, was examined in helmith parasites. Using the monoclonal antibody LM389, this antigen was detected in extracts from Taenia hydatigena, Mesocestoides vogae (syn corti), and Taenia crassiceps. No reactivity was observed in Thysanosoma spp., Dipylidium caninum, Fasciola hepatica, and Nyppostrongylus brasiliensis. On the basis of their electrophoretic mobility, different patterns of Tk-bearing glycoproteins were observed among T. hydatigena, M. corti and T. crassiceps by immunoblotting, with certain components resolved as broad bands typical of mucin-like glycoproteins. Most Tk-reactive material remained in the 0.6 N perchloric acid-soluble fraction, confirming that Tk epitopes are carried by mucin-type glycoproteins. Immunohistochemical analysis revealed that in T. hydatigena, Tk antigen is mainly expressed in the tegument, whereas in M. corti the reactivity was principally observed in the subtegumental parenchyma. The presence of a novel tumor-associated carbohydrate antigen in invertebrates, contributes to strengthen the notion that truncated mucin-type O-glycosylation is a normal phenomenon in parasitic worms and may help identify new biological characteristics of helminth parasites.

AB - Expression of Tk antigen, a truncated carbohydrate antigen, was examined in helmith parasites. Using the monoclonal antibody LM389, this antigen was detected in extracts from Taenia hydatigena, Mesocestoides vogae (syn corti), and Taenia crassiceps. No reactivity was observed in Thysanosoma spp., Dipylidium caninum, Fasciola hepatica, and Nyppostrongylus brasiliensis. On the basis of their electrophoretic mobility, different patterns of Tk-bearing glycoproteins were observed among T. hydatigena, M. corti and T. crassiceps by immunoblotting, with certain components resolved as broad bands typical of mucin-like glycoproteins. Most Tk-reactive material remained in the 0.6 N perchloric acid-soluble fraction, confirming that Tk epitopes are carried by mucin-type glycoproteins. Immunohistochemical analysis revealed that in T. hydatigena, Tk antigen is mainly expressed in the tegument, whereas in M. corti the reactivity was principally observed in the subtegumental parenchyma. The presence of a novel tumor-associated carbohydrate antigen in invertebrates, contributes to strengthen the notion that truncated mucin-type O-glycosylation is a normal phenomenon in parasitic worms and may help identify new biological characteristics of helminth parasites.

KW - 0.5% gelatin, 0.1% Tween 20 in phosphate buffered saline (PBS/TG)

KW - Bovine serum albumin (BSA)

KW - Carbohydrate cryptic antigen

KW - Carbohydrate cryptic antigens

KW - Deoxycholic acid (DOC)

KW - Ethylenediaminetetracetic acid (EDTA)

KW - Helminth parasites

KW - Monoclonal antibody (MAb)

KW - Mucin-type tumor markers

KW - N-acetylgalactosamine (GalNAc)

KW - O-glycosylation

KW - Perchloric acid (PCA)

KW - Phenylmethanesulfonyl fluoride (PMSF)

KW - Phosphate buffered saline (PBS) (composition: 137 mM NaCl, 3 mM KCl, and 15 mM Na/K-phosphate buffer, pH 7.4)

KW - Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)

KW - Tk antigen

KW - Tris-buffered saline (TBS) (composition: 150 mM NaCl, 50 mM Tris, pH 7.5)

UR - https://www.scopus.com/pages/publications/34247116966

U2 - 10.1016/j.exppara.2006.12.009

DO - 10.1016/j.exppara.2006.12.009

M3 - Artículo

C2 - 17300782

AN - SCOPUS:34247116966

SN - 0014-4894

VL - 116

SP - 129

EP - 136

JO - Experimental Parasitology

JF - Experimental Parasitology

IS - 2

ER -

Characterization of the carcinoma-associated Tk antigen in helminth parasites

Abstract

Keywords

UN SDGs

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