A novel clinically relevant animal model for studying galectin-3 and its ligands during colon carcinogenesis

Marcelo Hill, Daniel Mazal, Verónica Andrea Biron, Laura Pereira, Luis Ubillos, Edgardo Berriel, Hafiz Ahmed, Teresa Freire, Mariella Rondán, Gerardo R. Vasta, Fu Tong Liu, María Mercedes Iglesias, Eduardo Osinaga

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

15 Citas (Scopus)

Resumen

Galectin-3 (Gal-3) is a multifunctional protein that plays different roles in cancer biology. To better understand the role of Gal-3 and its ligands during colon carcinogenesis, we studied its expression in tumors induced in rats treated with 1,2-dimethylhydrazine (DMH) and in human tissues. Normal colon from untreated rats showed no staining using two specific monoclonal antibodies. In contrast, morphologically normal colon from DMH-treated rats and dysplastic aberrant crypt foci were strongly stained, indicating that increased Gal-3 expression is an early event during the neoplastic transformation in colon cells. Gal-3 was weakly expressed in adenocarcinomas. Overall, the Gal-3 expression pattern observed in the DMH rat model closely resembles that displayed by human colon stained with the same antibodies. We also found that Gal-3 phosphorylation diminishes in serines while increasing in tyrosines during rat colon carcinogenesis. Finally, we showed that Gal-3-ligands expression is strikingly similar in rat and human malignant colon and in non-malignant tissues. In conclusion, the DMH-induced rat colon cancer model displays expression patterns of Gal-3 and its ligands very similar to those observed in human samples. This animal model should contribute to clarifying the role of Gal-3 in colon carcinogenesis and also to finding effective preventive cancer agents based on Gal-3 targeting.

Idioma originalInglés
Páginas (desde-hasta)553-565
Número de páginas13
PublicaciónJournal of Histochemistry and Cytochemistry
Volumen58
N.º6
DOI
EstadoPublicada - jun. 2010
Publicado de forma externa

Huella

Profundice en los temas de investigación de 'A novel clinically relevant animal model for studying galectin-3 and its ligands during colon carcinogenesis'. En conjunto forman una huella única.

Citar esto