TY - JOUR
T1 - Characterization of the carcinoma-associated Tk antigen in helminth parasites
AU - Ubillos, Luis
AU - Medeiros, Andrea
AU - Cancela, Martín
AU - Casaravilla, Cecilia
AU - Saldaña, Jenny
AU - Domínguez, Laura
AU - Carmona, Carlos
AU - Le Pendu, Jacques
AU - Osinaga, Eduardo
PY - 2007/6
Y1 - 2007/6
N2 - Expression of Tk antigen, a truncated carbohydrate antigen, was examined in helmith parasites. Using the monoclonal antibody LM389, this antigen was detected in extracts from Taenia hydatigena, Mesocestoides vogae (syn corti), and Taenia crassiceps. No reactivity was observed in Thysanosoma spp., Dipylidium caninum, Fasciola hepatica, and Nyppostrongylus brasiliensis. On the basis of their electrophoretic mobility, different patterns of Tk-bearing glycoproteins were observed among T. hydatigena, M. corti and T. crassiceps by immunoblotting, with certain components resolved as broad bands typical of mucin-like glycoproteins. Most Tk-reactive material remained in the 0.6 N perchloric acid-soluble fraction, confirming that Tk epitopes are carried by mucin-type glycoproteins. Immunohistochemical analysis revealed that in T. hydatigena, Tk antigen is mainly expressed in the tegument, whereas in M. corti the reactivity was principally observed in the subtegumental parenchyma. The presence of a novel tumor-associated carbohydrate antigen in invertebrates, contributes to strengthen the notion that truncated mucin-type O-glycosylation is a normal phenomenon in parasitic worms and may help identify new biological characteristics of helminth parasites.
AB - Expression of Tk antigen, a truncated carbohydrate antigen, was examined in helmith parasites. Using the monoclonal antibody LM389, this antigen was detected in extracts from Taenia hydatigena, Mesocestoides vogae (syn corti), and Taenia crassiceps. No reactivity was observed in Thysanosoma spp., Dipylidium caninum, Fasciola hepatica, and Nyppostrongylus brasiliensis. On the basis of their electrophoretic mobility, different patterns of Tk-bearing glycoproteins were observed among T. hydatigena, M. corti and T. crassiceps by immunoblotting, with certain components resolved as broad bands typical of mucin-like glycoproteins. Most Tk-reactive material remained in the 0.6 N perchloric acid-soluble fraction, confirming that Tk epitopes are carried by mucin-type glycoproteins. Immunohistochemical analysis revealed that in T. hydatigena, Tk antigen is mainly expressed in the tegument, whereas in M. corti the reactivity was principally observed in the subtegumental parenchyma. The presence of a novel tumor-associated carbohydrate antigen in invertebrates, contributes to strengthen the notion that truncated mucin-type O-glycosylation is a normal phenomenon in parasitic worms and may help identify new biological characteristics of helminth parasites.
KW - 0.5% gelatin, 0.1% Tween 20 in phosphate buffered saline (PBS/TG)
KW - Bovine serum albumin (BSA)
KW - Carbohydrate cryptic antigen
KW - Carbohydrate cryptic antigens
KW - Deoxycholic acid (DOC)
KW - Ethylenediaminetetracetic acid (EDTA)
KW - Helminth parasites
KW - Monoclonal antibody (MAb)
KW - Mucin-type tumor markers
KW - N-acetylgalactosamine (GalNAc)
KW - O-glycosylation
KW - Perchloric acid (PCA)
KW - Phenylmethanesulfonyl fluoride (PMSF)
KW - Phosphate buffered saline (PBS) (composition: 137 mM NaCl, 3 mM KCl, and 15 mM Na/K-phosphate buffer, pH 7.4)
KW - Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
KW - Tk antigen
KW - Tris-buffered saline (TBS) (composition: 150 mM NaCl, 50 mM Tris, pH 7.5)
UR - http://www.scopus.com/inward/record.url?scp=34247116966&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2006.12.009
DO - 10.1016/j.exppara.2006.12.009
M3 - Artículo
C2 - 17300782
AN - SCOPUS:34247116966
SN - 0014-4894
VL - 116
SP - 129
EP - 136
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 2
ER -